Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Yoshida, Fumiyo*; Matsumura, Akira*; Yamamoto, Tetsuya*; Kumada, Hiroaki; Nakai, Kei*
Cancer Letters, 215(1), p.61 - 67, 2004/11
Times Cited Count：13 Percentile：22.4(Oncology)Sodium borocaptate (BSH) is widely used for Boron Neutron Capture Therapy (BNCT) of brain tumors. However, the mechanism of uptake by the tumor remains unclear. We investigated the sulfhydryl moiety of this compound. Down regulation of glutathione (GSH) by buthionine sulfoximine (BSO) in cultured cells resulted in increase of BSH uptake (7.9 %-36.5 %) compared to control group and subsequently cytocidal effect of neutron irradiation also increased. The radiation damage by 
-ray irradiation was suppressed by enhancement of BSH uptake. These findings suggested that modulation of GSH induce enhancement effect of B(n,
) reaction and protective effect of secondary -ray in BNCT.
is involved in the accumulation of both anthocyanins and proanthocyanidins in 
Kitamura, Satoshi; Shikazono, Naoya; Tanaka, Atsushi
Plant Journal, 37(1), p.104 - 114, 2004/01
Times Cited Count：383 Percentile：99.06(Plant Sciences)Flavonoid compounds such as anthocyanins and proanthocyanidins (PAs; so-called condensed tannins) must be transported from the site of synthesis in the cytosol to the vacuoles. Novel Arabidopsis mutants, transparent testa 19 (tt19), which were induced by ion beam irradiation, showed a great reduction of anthocyanin pigments in the vegetative parts as well as brown pigments in the seed coat. The TT19 gene was isolated by chromosome walking and the gene candidate approach, and was shown to be a member of the Arabidopsis glutathione S-transferase (GST) gene family. Heterologous expression of a putative ortholog, petunia AN9, in tt19 complemented the anthocyanin accumulation but not the brown pigmentation in the seed coat. This suggests that the TT19 gene is required for vacuolar uptake of anthocyanins into vacuoles, but that it has also a different function from AN9. The depositional pattern of PA precursors in the mutant was different from that in the wild type. These results indicate that TT19 participates in the PA pathway as well as the anthocyanin pathway of Arabidopsis.
